Prestained Protein Ladder - Extra broad molecular weight (5 (2024)

Overview

• Product name

  Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa)
  See all Prestained Protein Ladder reagents

  See Also

  Protein Standards & Ladders - KRWestern Blotting - USProtein Standards & Ladders - US

• Tested applications

  Suitable for: WB, SDS-PAGEmore details

• General notes

  Prestained Protein Ladder ab116029 is a three-color protein standard with 13 pre-stained proteins covering a wide range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).

  This product is also sold by Expedeon, an Abcam company as RunBlue™ TriColor Prestained Protein Ladder: NXA6050. This product was previously calledPrism Ultra Protein Ladder (3.5 - 245 kDa).

  This prestained protein ladder is designed for monitoring protein separation during SDS polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.

  Key product features:

  • Broad range: 3.5-245 kDa
  • Ready-to-use: Supplied in a loading buffer for direct loading on gels.
  • Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.
  • Sharp bands
  • Recommended loading: ~1.5 - 2.5µl

  Review other protein ladders in theunstained and prestained protein ladder guide.

Properties

• Form

  Liquid

• Storage instructions

  Store at +4°C short term (1-2 weeks). Store at -20°C.

• Storage buffer

  pH: 7.5
  Constituents: 0.44% Tris citrate/phosphate, 0.02% Urea, 2% Sodium lauryl sulfate, 0.02% DTT, 15% Glycerol

• Concentration information loading...

• Research areas

  • Kits/ Lysates/ Other
  • Tools and Reagents
  • Western Blot Tools and Reagents
  • Protein ladders

• Alternative names

  • Molecular weight marker
  • Prism protein ladder
  • SDS PAGE protein ladder prestained

  see all

• SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa) (ab116029)

  Gel 1: Tris-Glycine (4-15%) SDS buffer; Gel 2: Bis-Tris (4-12%) MOPS buffer; Gel 3: Bis-Tris (4-12%) MES buffer. Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.

• SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa) (ab116029)

  Gel 1: Tris-Glycine (~4-20%), Gel 2: Bis-Tris (10%) MES buffer. Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

• SDS download

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• Datasheet download

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ab116029 has been referenced in 3 publications.

• Idrees M et al. SHP2 Nuclear/Cytoplasmic Trafficking in Granulosa Cells Is Essential for Oocyte Meiotic Resumption and Maturation. Front Cell Dev Biol 8:611503 (2020). PubMed: 33553147
• Idrees M et al. PTPN11 (SHP2) Is Indispensable for Growth Factors and Cytokine Signal Transduction During Bovine Oocyte Maturation and Blastocyst Development. Cells 8:N/A (2019). PubMed: 31635340
• Idrees M et al. The PPARd Agonist GW501516 Improves Lipolytic/Lipogenic Balance through CPT1 and PEPCK during the Development of Pre-Implantation Bovine Embryos. Int J Mol Sci 20:N/A (2019). PubMed: 31810173